Genotyping and DNA-RNA Analysis Core

Taste and olfactory receptor genes are among the most variable in the human genome and the focus of this core is to offer expertise in the analysis of genetic variation in these and related genes.

DNA and RNA isolation, purification, and quantification

To quantify DNA or RNA, we have a Nanodrop (a quick way to measure one-microliter samples for DNA, RNA, and protein concentration), an Agilent Tape Station for the measurement of RNA, gel electrophoresis to semi-quantify DNA and RNA, and pico-green which is a method to measure double-stranded genomic DNA. We also have a gel documentation system to photograph agarose gels. We conduct fee-for-service extraction of genomic DNA from human saliva especially in preparation for bitter receptor genotyping (see below).

Genotyping

We have a StepOne real-time PCR instrument and a QuantStudio 12K Flex Open Array for real-time PCR using single-assay dye-labeled primers and probes as well as array-based genotyping. Specifically, we have an array to measure multiple alleles in taste receptor genes. The QuantStudio instrument was the result of an NIH S10 grant (OD018125).

TAS2R38 genotyping

We conduct fee-for-service genotyping on human genomic DNA samples to determine the haplotypes that explain person-to-person differences in the ability to taste the bitter chemical phenylthiocarbamide and chemically related compounds, e.g., propylthiouracil or goitrin.

Typhoon 9400

We also have a multimodal, high-throughput imaging system that unites proven storage phosphor autoradiography technology with four-color fluorescence labeling and enhanced chemiluminescence detection for the quantitative analysis of biomolecules in DNA, RNA, and protein samples.